5L5M
Plexin A4 full extracellular region, domains 1 to 7 modeled, data to 8 angstrom, spacegroup P4(3)2(1)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 271.480, 271.480, 251.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.126 - 8.000 |
| R-factor | 0.3743 |
| Rwork | 0.373 |
| R-free | 0.39500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l56 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.473 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.126 | |
| High resolution limit [Å] | 8.000 | 8.000 |
| Rmerge | 0.130 | 0.894 |
| Number of reflections | 10298 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.5 | 0.8 M ammonium sulfate, 0.4 M Dimethyl (2-hydroxyethyl) ammonium propane sulfonate (NDSB-211), 100 mM TRIS, pH 8.0 |
