5L5M

Plexin A4 full extracellular region, domains 1 to 7 modeled, data to 8 angstrom, spacegroup P4(3)2(1)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	271.480, 271.480, 251.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.126 - 8.000
R-factor	0.3743
Rwork	0.373
R-free	0.39500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l56
RMSD bond length	0.019
RMSD bond angle	2.473
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.126
High resolution limit [Å]	8.000	8.000
Rmerge	0.130	0.894
Number of reflections	10298
<I/σ(I)>	14.8
Completeness [%]	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.5	0.8 M ammonium sulfate, 0.4 M Dimethyl (2-hydroxyethyl) ammonium propane sulfonate (NDSB-211), 100 mM TRIS, pH 8.0