5L43
Structure of K26-DCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.470, 66.936, 101.786 |
| Unit cell angles | 97.34, 90.86, 117.31 |
Refinement procedure
| Resolution | 58.810 - 1.800 |
| R-factor | 0.17824 |
| Rwork | 0.177 |
| R-free | 0.20023 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1Y79 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.415 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 0.624 |
| Number of reflections | 124841 | |
| <I/σ(I)> | 7.3 | 1.8 |
| Completeness [%] | 95.0 | 62.4 |
| Redundancy | 3.2 | 2.8 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M MgCl2, 0.1M sodium cacodylate,20% PEG 8000 |
