5L3V

Structure of the crenarchaeal SRP54 GTPase bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 32 2 1
Unit cell lengths	92.168, 92.168, 148.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.136 - 2.300
R-factor	0.1782
Rwork	0.176
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l3s
RMSD bond length	0.009
RMSD bond angle	1.163
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.140	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	33058
<I/σ(I)>	22.3
Completeness [%]	99.8
Redundancy	19.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M CHES pH 6.0, 15 % (w/v) PEG 8000, 0.2 M ammonium sulfate