5L3S

Structure of the GTPase heterodimer of crenarchaeal SRP54 and FtsY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	51.196, 199.672, 124.869
Unit cell angles	90.00, 92.95, 90.00

Refinement procedure

Resolution	37.870 - 1.900
R-factor	0.1741
Rwork	0.173
R-free	0.21620
RMSD bond length	0.008
RMSD bond angle	1.016
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.870
High resolution limit [Å]	1.900
Number of reflections	191335
<I/σ(I)>	11.2
Completeness [%]	97.3
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES pH 5.8, 1.26 M ammonium sulfate