5L3R

Structure of the GTPase heterodimer of chloroplast SRP54 and FtsY from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	89.790, 74.750, 107.210
Unit cell angles	90.00, 91.14, 90.00

Refinement procedure

Resolution	44.886 - 2.500
R-factor	0.1751
Rwork	0.173
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l3s
RMSD bond length	0.008
RMSD bond angle	0.982
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.730	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	49064
<I/σ(I)>	9.5
Completeness [%]	99.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M magnesium formate, 20% (w/v) PEG 3350