5L3G

LSD1-CoREST1 in complex with polymyxin E (colistin)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	119.864, 179.490, 236.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.784 - 3.100
R-factor	0.1734
Rwork	0.173
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
RMSD bond length	0.014
RMSD bond angle	1.650
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.784	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.069
Number of reflections	46474
<I/σ(I)>	11.6	1.1
Completeness [%]	99.7	99.8
Redundancy	3.5	3.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.3 M Na/K Tartrate 100 mM ADA