5L37
The structure of the pentameric shell protein MSM0273 from the RMM microcompartment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-10 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.970, 69.970, 140.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.800 - 1.600 |
| R-factor | 0.1555 |
| Rwork | 0.154 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qw7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.875 |
| Number of reflections | 52789 | |
| <I/σ(I)> | 17.7 | 2.3 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 7.3 | 7.1 |
| CC(1/2) | 0.999 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 90 mM Na acetate, 1.8 M NaCl, 10mM TCEP |
