5L2E

Crystal structure of rat Glutamate receptor delta-2 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2016-04-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 32 2 1
Unit cell lengths	179.172, 179.172, 214.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.583 - 4.152
R-factor	0.2176
Rwork	0.211
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2VT3 and 5KC8
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	HKL-2000 (0.98.712)
Data scaling software	HKL-2000 (0.98.712)
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.220
High resolution limit [Å]	4.150	4.150
Rmerge	0.157	0.795
Number of reflections	30494
<I/σ(I)>	8.9	1.6
Completeness [%]	100.0	100
Redundancy	4.2	3.9
CC(1/2)		0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	294	0.1 M Sodium cacodylate pH 6.6, 1.3 M Ammonium dihydrogen phosphate