5L2D
Streptococcal surface adhesin - CshA NR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 173.929, 173.929, 104.071 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.010 - 2.660 |
| R-factor | 0.20435 |
| Rwork | 0.201 |
| R-free | 0.26353 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.826 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.010 | 2.800 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.107 | 1.477 |
| Number of reflections | 27284 | |
| <I/σ(I)> | 19.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.8 | 16.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Na/K tartrate, MMT buffer [DL-malic acid, MES and Tris base in the molar ratios 1:2:2] (pH 5.0), 22% PEG 3350. |
