5L25
Crystal Structure of Arabidopsis thaliana Bor1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 184.557, 184.557, 89.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.070 - 4.110 |
| R-factor | 0.3602 |
| Rwork | 0.359 |
| R-free | 0.39149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yzf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.070 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.176 | |
| Number of reflections | 11319 | |
| <I/σ(I)> | 19.3 | 1.1 |
| Completeness [%] | 96.3 | 87.6 |
| Redundancy | 12 | 7.7 |
| CC(1/2) | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 100mM sodium citrate pH 5.8, 300mM lithium sulfate, 10.5% PEG3350 |
