5L22
PrtD T1SS ABC transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0007 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.672, 97.938, 179.823 |
| Unit cell angles | 90.00, 100.47, 90.00 |
Refinement procedure
| Resolution | 33.536 - 3.150 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.27580 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.736 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 3.270 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.107 | 0.809 |
| Number of reflections | 35413 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 97.4 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 16-21% PEG 1000, 100-400 mM (NH4)2SO4, with 10 mM MES pH 6.5 |
