5L1M
CASKIN2 SAM domain tandem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 96.460, 96.460, 119.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.509 - 2.751 |
| R-factor | 0.2471 |
| Rwork | 0.245 |
| R-free | 0.26490 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.510 |
| High resolution limit [Å] | 2.750 |
| Rmerge | 0.077 |
| Number of reflections | 9004 |
| <I/σ(I)> | 56.8 |
| Completeness [%] | 100.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 | 0.1 M Tris pH 7.5, 2.4 M sodium formate, 5 mM DTT |
