5L19
Crystal Structure of a human FasL mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2016-02-10 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 52.690, 52.690, 87.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.16775 |
| Rwork | 0.165 |
| R-free | 0.21602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4msv |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.999 |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.190 | 0.942 |
| Number of reflections | 9993 | |
| <I/σ(I)> | 13.3 | 3.1 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 9.4 | 8.6 |
| CC(1/2) | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 0.2M Ammonium Sulfate, 0.1M Bis-Tris:HCl, pH6.5, 20% (W/V) PEG3350, 0.01M Zinc Chloride |
