5L0T

human POGLUT1 in complex with EGF(+) and UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-23
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	71.660, 75.200, 82.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.984 - 1.430
R-factor	0.157
Rwork	0.156
R-free	0.17340
RMSD bond length	0.009
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.430	1.430
Rmerge	0.079	1.584
Number of reflections	83350
<I/σ(I)>	14.83	1.6
Completeness [%]	100.0	100
Redundancy	8.1	7.7
CC(1/2)	0.998	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% PEG5000 MME, 50 mM MES pH 6.5, 10mM CaCl2, 250mM NaCl, 5% glycerol