5L0R

human POGLUT1 in complex with Notch1 EGF12 and UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	70.580, 73.560, 83.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.448 - 1.500
R-factor	0.15
Rwork	0.149
R-free	0.17070
RMSD bond length	0.013
RMSD bond angle	1.299
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.053	0.864
Number of reflections	68848
<I/σ(I)>	19.19	1.64
Completeness [%]	98.0	91
Redundancy	5.7	4.7
CC(1/2)	0.999	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% PEG5000 MME, 50 mM MES pH 6.5, 2mM CaCl2, 250mM NaCl, 5% glycerol