5L0A
Human muscle fructose-1,6-bisphosphatase E69Q mutant in active R-state in complex with fructose-1,6-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-27 |
Detector | RAYONIX SX-165mm |
Wavelength(s) | 0.8943 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 72.446, 72.446, 233.954 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.027 - 2.302 |
R-factor | 0.2203 |
Rwork | 0.215 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5et5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.223 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.030 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.076 | 0.622 |
Number of reflections | 13949 | |
<I/σ(I)> | 3.49 | 2 |
Completeness [%] | 93.9 | 69.5 |
Redundancy | 8.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 10mM magnesium chloride, 2M sodium chloride, 10%PEG6000, 10mM Tris |