5KZS

Listeria monocytogenes internalin-like protein lmo2027

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	I 4
Unit cell lengths	157.776, 157.776, 36.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.23031
Rwork	0.228
R-free	0.27907
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hl3
RMSD bond length	0.009
RMSD bond angle	1.469
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.571
Number of reflections	20009
<I/σ(I)>	16.6	1.9
Completeness [%]	98.6	99.6
Redundancy	6.2	4.5
CC(1/2)		0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	Protein: 12.6 mg/ml, 0.5 M NaCl, 10 mM Tris pH 8.3, 1 mM TCEP Crystallization condition: Classics II D4 (Qiagen), 0.1 M Citric acid (pH 3.5) and 25% (w/v) PEG 3350