5KZL

Structure of Heme Oxygenase from Leptospira interrogans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-30
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	37.640, 58.790, 86.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.700 - 1.730
R-factor	0.186
Rwork	0.184
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1we1
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.700	1.830
High resolution limit [Å]	1.730	1.730
Rmerge	0.129	1.178
Number of reflections	20079
<I/σ(I)>	20.8	2.64
Completeness [%]	96.7	89.3
Redundancy	13.2	10
CC(1/2)	0.998	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	24% PEG 4000 0,1 M sodium citrate (pH 6.0) 0,2 M ammonium acetate