5KZK

Crystal Structure of rRNA methyltransferase from Sinorhizobium meliloti

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	65.820, 84.302, 112.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.360 - 2.280
R-factor	0.20867
Rwork	0.206
R-free	0.25863
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.075
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	67.360
High resolution limit [Å]	2.280
Number of reflections	28693
<I/σ(I)>	46.98
Completeness [%]	98.0
Redundancy	7.9
CC(1/2)	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	5mM Cobalt chloride , 5mM Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 9%(wt/vol) polyethylene glycol 3,350