5KZB

Crystal structure of the Rous sarcoma virus matrix protein (aa 2-102). Space group I4122

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2007-10-24
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	I 41 2 2
Unit cell lengths	67.392, 67.392, 220.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.700 - 3.200
R-factor	0.20424
Rwork	0.202
R-free	0.24851
RMSD bond length	0.013
RMSD bond angle	1.695
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.700
High resolution limit [Å]	3.200
Rmerge	0.132
Number of reflections	4535
<I/σ(I)>	22.2
Completeness [%]	99.9
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.1	291.15	0.6 M Malonic acid /KOH pH 9.1, 0.1 M Boric acid /KOH pH 9.1