5KYW

crystal structure of Sec23 and TANGO1 peptide3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	101.625, 141.346, 151.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.512 - 3.200
R-factor	0.2124
Rwork	0.210
R-free	0.25690
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.512
High resolution limit [Å]	3.200
Number of reflections	36535
<I/σ(I)>	10.6
Completeness [%]	99.9
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM HEPES pH 7.5, 5.5 % (w/v) PEG 4000, 100 mM MgSO4