5KYM
Crystal Structure of the 1-acyl-sn-glycerophosphate (LPA) acyltransferase, PlsC, from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-07 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 173.836, 173.836, 173.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.460 - 2.800 |
| R-factor | 0.22359 |
| Rwork | 0.219 |
| R-free | 0.27703 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.009 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.460 | 2.901 |
| High resolution limit [Å] | 2.800 | 2.802 |
| Rmerge | 0.188 | |
| Number of reflections | 21613 | |
| <I/σ(I)> | 10.18 | 0.6 |
| Completeness [%] | 99.9 | 99.58 |
| Redundancy | 9 | 4.6 |
| CC(1/2) | 0.996 | 0.167 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18-22% (w/v) polyethylene glycol 3350 (PEG3350), 0.1 M HEPES, and 0.2 M MgCl 2 |
