5KYG
RAWV_CTD (Loop Structure) of 16S/23S 2'-O-methyltransferase TlyA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-04-18 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.922, 70.922, 79.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.040 - 1.900 |
| R-factor | 0.17843 |
| Rwork | 0.173 |
| R-free | 0.22399 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eov |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.526 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.040 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.121 | 0.762 |
| Number of reflections | 16678 | |
| <I/σ(I)> | 45.8 | 3.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.8 | 9.1 |
| CC(1/2) | 0.928 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 8% Tascimate pH 8.0, 20% PEG 3350 |
