5KY6
Human muscle fructose-1,6-bisphosphate aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.511, 57.253, 164.016 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 46.721 - 1.941 |
| R-factor | 0.1867 |
| Rwork | 0.186 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ald |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.166 | 0.721 |
| Number of reflections | 112653 | |
| <I/σ(I)> | 10.01 | 2.85 |
| Completeness [%] | 99.0 | 94.4 |
| Redundancy | 5.68 | 5.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 1.6M ammonium citrate tribasic |
