5KXZ

Hen Egg White Lysozyme at 278K, Data set 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	278
Detector technology	CCD
Collection date	2013-07-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.21549
Spacegroup name	P 43 21 2
Unit cell lengths	79.159, 79.159, 37.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.413 - 1.200
R-factor	0.1832
Rwork	0.182
R-free	0.21080
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2blx
RMSD bond length	0.022
RMSD bond angle	2.187
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.413	31.413	1.260
High resolution limit [Å]	1.198	3.790	1.200
Rmerge		0.092	2.638
Rmeas	0.147	0.107	3.115
Rpim	0.073	0.052	1.600
Total number of observations	124191	4186	14188
Number of reflections	35811
<I/σ(I)>	6.7	18.4	0.5
Completeness [%]	93.8	93.2	86.5
Redundancy	3.5	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	CRYSTALS WERE GROWN BY MIXING EQUAL VOLUMES OF WELL SOLUTION (1.0 M SODIUM CHLORIDE, 50 MM SODIUM ACETATE PH 4.5) AND PROTEIN (40-60 MG/ML)