5KXF

Crystal structure of the SPOC domain of the Arabidopsis flowering regulator FPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	108.151, 108.151, 34.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.910 - 2.700
R-factor	0.19786
Rwork	0.192
R-free	0.25395
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.927
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.910	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.105	0.453
Number of reflections	6505
<I/σ(I)>	24.11	6.33
Completeness [%]	100.0	100
Redundancy	12	12.3
CC(1/2)		0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	magnesium sulfate, PEG 3350