5KXF
Crystal structure of the SPOC domain of the Arabidopsis flowering regulator FPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 108.151, 108.151, 34.208 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.910 - 2.700 |
| R-factor | 0.19786 |
| Rwork | 0.192 |
| R-free | 0.25395 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.910 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.105 | 0.453 |
| Number of reflections | 6505 | |
| <I/σ(I)> | 24.11 | 6.33 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12.3 |
| CC(1/2) | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | magnesium sulfate, PEG 3350 |
