5KWB
Crystal Structure of the Receptor Binding Domain of the Spike Glycoprotein of Human Betacoronavirus HKU1 (HKU1 1A-CTD, 1.9 angstrom, molecular replacement)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97776 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.556, 183.800, 63.342 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.248 - 1.910 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.20060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gnb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.046 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.112 | 0.750 |
Number of reflections | 75702 | |
<I/σ(I)> | 9.37 | 1.6 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 3.45 | 3.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris, 24%PEG4000, 0.2M MgCl2 |