5KVM
Extracellular region of mouse GPR56/ADGRG1 in complex with FN3 monobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2014-08-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 65 |
| Unit cell lengths | 120.336, 120.336, 72.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.391 - 2.449 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.26330 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.611 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.391 | 2.490 |
| High resolution limit [Å] | 2.449 | 2.450 |
| Rmerge | 0.035 | 0.421 |
| Number of reflections | 22286 | |
| <I/σ(I)> | 19.75 | 1.5 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 5.7 | 5.7 |
| CC(1/2) | 0.923 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 80 mM sodium acetate pH 4.6, 19.5% glycerol, 16.9% PEG 600, 7.6% PEG 1000 |
