5KVM

Extracellular region of mouse GPR56/ADGRG1 in complex with FN3 monobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2014-08-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 65
Unit cell lengths	120.336, 120.336, 72.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.391 - 2.449
R-factor	0.2171
Rwork	0.215
R-free	0.26330
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.611
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.391	2.490
High resolution limit [Å]	2.449	2.450
Rmerge	0.035	0.421
Number of reflections	22286
<I/σ(I)>	19.75	1.5
Completeness [%]	99.9	99.5
Redundancy	5.7	5.7
CC(1/2)	0.923	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	80 mM sodium acetate pH 4.6, 19.5% glycerol, 16.9% PEG 600, 7.6% PEG 1000