5KVG

Zika specific antibody, ZV-67, bound to ZIKA envelope DIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-06-15
Detector	RDI CMOS_8M
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	73.110, 82.930, 93.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.970 - 1.400
R-factor	0.1498
Rwork	0.148
R-free	0.17870
RMSD bond length	0.006
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.970		1.160
High resolution limit [Å]	1.140	5.080	1.140
Rmerge	0.128	0.040
Number of reflections	150169
<I/σ(I)>	11.91	55
Completeness [%]	79.1	99.7	9.4
Redundancy	27.4
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium formate, 20% PEG 3350