5KVE

Zika specific antibody, ZV-48, bound to ZIKA envelope DIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-05-28
Detector	RDI CMOS_8M
Wavelength(s)	1.00004
Spacegroup name	C 2 2 21
Unit cell lengths	63.240, 87.980, 147.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.497 - 1.700
R-factor	0.1881
Rwork	0.187
R-free	0.21270
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.441		1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.059	0.021	0.989
Number of reflections	44574
<I/σ(I)>	18.76	61.04	2.04
Completeness [%]	98.9	94.6	100
Redundancy	14.5
CC(1/2)	0.999	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M Ammonium sulfate,15 %(w/v) PEG 4000