5KUT

hMiro2 C-terminal GTPase domain, GDP-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978720
Spacegroup name	C 2 2 21
Unit cell lengths	80.610, 122.760, 103.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.576 - 1.693
R-factor	0.219
Rwork	0.218
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0l
RMSD bond length	0.005
RMSD bond angle	0.640
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.800
High resolution limit [Å]	1.690	1.690
Rmerge	0.085
Number of reflections	57061
<I/σ(I)>	10.1
Completeness [%]	99.2
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	14 mg/mL protein, 0.2 M magnesium formate, 20% w/v PEG3350