5KU7

Crystal structure of the TIR domain from the Muscadinia rotundifolia disease resistance protein RPV1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-04-03
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 2 21 21
Unit cell lengths	41.855, 89.117, 113.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.947 - 2.300
R-factor	0.1853
Rwork	0.180
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4csr
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.947	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.091	0.927
Number of reflections	19672
<I/σ(I)>	19.6	2.2
Completeness [%]	100.0	100
Redundancy	7.2	7.4
CC(1/2)	0.990	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	16% PEG3350, 0.1M Tris pH 8.5 and 2% Tacsimate