5KU5

Crystal Structure of CusS Sensor Domain with Silver Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.036
Spacegroup name	P 2 21 21
Unit cell lengths	35.225, 96.289, 203.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.290 - 2.150
R-factor	0.2515
Rwork	0.250
R-free	0.28640
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.060
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.290
High resolution limit [Å]	2.150
Number of reflections	38599
<I/σ(I)>	6.3
Completeness [%]	99.0
Redundancy	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM Tris HCl pH 8.5 200 mM Ammonium acetate 25% PEG 3350