5KTV
Mis-pairing of unnatural base Z-G DNA duplex at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.928190 |
| Spacegroup name | H 3 |
| Unit cell lengths | 40.924, 40.924, 102.147 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.050 - 2.350 |
| R-factor | 0.18316 |
| Rwork | 0.180 |
| R-free | 0.23775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 476d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.480 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 2550 |
| <I/σ(I)> | 2.3 |
| Completeness [%] | 100.0 |
| Redundancy | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 100mM Sodium Chloride, 20mM Tris pH 8.5, 34% hexylene glycol, |
