5KTQ

LARGE FRAGMENT OF TAQ DNA POLYMERASE BOUND TO DCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	287
Spacegroup name	P 21 21 2
Unit cell lengths	109.400, 137.000, 45.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.217
Rwork	0.217
R-free	0.28000
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	24.200 *
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Rmerge	0.069 *
Total number of observations	62452 *
Number of reflections	21040
Completeness [%]	85.9
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9		pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	6 (%(w/v))
2	1	reservoir		50 (mM)
3	1	reservoir	Tris-HCl	100 (mM)