5KTD
FdhC with bound products: Coenzyme A and dTDP-3-amino-3,6-dideoxy-d-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-14 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 75.812, 75.812, 73.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.20419 |
| Rwork | 0.201 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kta |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.929 |
| Data reduction software | SAINT (7.06A) |
| Data scaling software | SADABS (2005/1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.108 |
| Number of reflections | 17505 |
| <I/σ(I)> | 21.1 |
| Completeness [%] | 99.7 |
| Redundancy | 29.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5 | 277 | 30% monomethylether poly(ethylene glycol) 5000, 200 mM LiCl, and 100 mM Homo-PIPES |
