5KTA
Apo FdhC- a nucleotide-linked sugar GNAT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-09 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 69.549, 69.549, 137.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| R-factor | 0.19762 |
| Rwork | 0.195 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lab Model from SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.846 |
| Data reduction software | SAINT (7.06A) |
| Data scaling software | SADABS (2005/1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.890 |
| Rmerge | 0.080 |
| Number of reflections | 16130 |
| <I/σ(I)> | 17.1 |
| Completeness [%] | 98.0 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.5 M AmSo4, 2% DMSO, 100 mM HEPPS |
