5KSO

hMiro1 C-domain GDP-Pi Complex P3121 Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978590
Spacegroup name	P 31 2 1
Unit cell lengths	65.510, 65.510, 82.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.369 - 2.250
R-factor	0.1785
Rwork	0.175
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0l
RMSD bond length	0.003
RMSD bond angle	0.680
Data reduction software	MOSFLM (7.1.0)
Data scaling software	Aimless (0.3.8)
Phasing software	PHENIX (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.500
High resolution limit [Å]	2.250	2.250
Rmerge	0.083
Number of reflections	10140
<I/σ(I)>	83
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	294	19 mg/mL protein, 5 mM magnesium chloride, 0.1 M SPG buffer, pH 5.0, 25% w/v PEG1500, GDP observed in the structure was carried through from the expression system during purification