5KSB

T15-DQ8.5-glia-gamma1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	115.890, 125.050, 165.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.000 - 2.900
R-factor	0.221
Rwork	0.219
R-free	0.25400
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	85.000
High resolution limit [Å]	2.900
Number of reflections	53672
<I/σ(I)>	8.7
Completeness [%]	99.4
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	8% Tacsimate pH 7.5, 20% PEG3350