5KS2

RAWV_CTD (Helix form) of 16S/23S 2'-O-methyltransferase TlyA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2016-02-21
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	67.852, 67.852, 79.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.600 - 2.180
Rwork	0.193
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eov
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.600	2.240
High resolution limit [Å]	2.180	2.180
Rmerge	0.319	0.984
Number of reflections	10172
<I/σ(I)>	19.2	4.7
Completeness [%]	99.9	99.9
Redundancy	13.2	5.6
CC(1/2)	0.558	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.3 M Hepes pH 7.5 2.8M NaCl