5KQP
Crystal structure of Apo-form LMW-PTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.957, 55.453, 96.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.029 - 2.052 |
R-factor | 0.1963 |
Rwork | 0.193 |
R-free | 0.26630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5pnt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.066 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.050 |
Rmerge | 0.102 |
Number of reflections | 11281 |
<I/σ(I)> | 25.5 |
Completeness [%] | 99.9 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals grew under the following condition: 30% PEGME 5000, 0.2 M ammonium sulfate, 100 mM MES buffer, pH=6.5. Then crystal were transferred to buffer containing 30% PEGME 5000, 0.2 M ammonium sulfate, 100 mM Bis-Tris, pH=6.5 to deplete the bound MES. |