5KOF

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and Acyl Carrier Protein, AcpP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	59.330, 103.920, 83.400
Unit cell angles	90.00, 110.61, 90.00

Refinement procedure

Resolution	48.978 - 2.400
R-factor	0.2105
Rwork	0.209
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vb9
RMSD bond length	0.006
RMSD bond angle	1.508
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2063: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.978	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.050	0.395
Number of reflections	37072
<I/σ(I)>	7.27	1.76
Completeness [%]	100.0	100
Redundancy	2	1.9
CC(1/2)	0.996	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	298	0.2 M Ammonium acetate, 0.1 M Sodium acetate pH 5.4, 20% PEG 4000