5KOA
Structure of Escherichia coli ZapD bound to the C-terminal tail of FtsZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.322, 51.912, 79.146 |
| Unit cell angles | 90.00, 90.68, 90.00 |
Refinement procedure
| Resolution | 79.140 - 2.670 |
| R-factor | 0.2337 |
| Rwork | 0.230 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oez |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.455 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.141 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.480 | |
| Number of reflections | 15706 | |
| <I/σ(I)> | 10.7 | 1.6 |
| Completeness [%] | 94.1 | 73.1 |
| Redundancy | 3 | 2.1 |
| CC(1/2) | 0.997 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 8000, 100 mM Imidazole, MPD |
