5KNZ

Human Islet Amyloid Polypeptide Segment 19-SGNNFGAILSS-29 with Early Onset S20G Mutation Determined by MicroED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2014-11-11
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 21 21 21
Unit cell lengths	4.780, 18.600, 70.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	1.900 - 1.900
R-factor	0.2318
Rwork	0.228
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.233
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.400	35.400	2.130
High resolution limit [Å]	1.900	4.250	1.900
Rmerge	0.106	0.061	0.150
Rmeas	0.130	0.073	0.191
Total number of observations	1380
Number of reflections	548	59	115
<I/σ(I)>	5.65	7.92	3.65
Completeness [%]	82.9	81.9	64.6
CC(1/2)	0.989	0.996	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.4	295	1 mmol SGNNFGAILSS in 1 L phosphate-buffered saline with 1% DMSO incubated under quiescent conditions overnight