5KNK

Lipid A secondary acyltransferase LpxM from Acinetobacter baumannii with catalytic residue substitution (E127A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	CCD
Collection date	2016-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	59.300, 145.470, 87.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.735 - 1.900
R-factor	0.2265
Rwork	0.225
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kn7
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	72.740
High resolution limit [Å]	1.900
Number of reflections	30262
<I/σ(I)>	7.95
Completeness [%]	100.0
Redundancy	1.9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	200 mM KBr 2.2 M (NH4)2SO4