5KN3
Recombinant bovine skeletal calsequestrin, low-Ca2+ form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.393, 146.060, 110.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.237 - 1.849 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3trp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | HKL-2000 (v712) |
| Data scaling software | HKL-2000 (v712) |
| Phasing software | PHENIX (1.10_2155) |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.237 | 1.915 |
| High resolution limit [Å] | 1.849 | 1.849 |
| Rmerge | 0.070 | 0.820 |
| Number of reflections | 41309 | |
| <I/σ(I)> | 13.4 | 2.72 |
| Completeness [%] | 100.0 | 97 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, 27.5 % 2-methyl-2,4-pentanediol, 0.2 M NaCl |
