5KMU
CTX-M-9 beta lactamase mutant - T165W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.97121 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.743, 42.212, 68.035 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 32.950 - 1.800 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.18426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.486 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.950 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 20959 |
| <I/σ(I)> | 17.4 |
| Completeness [%] | 95.5 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 294 | 0.7 M potassium phosphate, 18% PEG 3350 |
