5KLU
Crystal Structure of a Domain-swapped Dimer of Yeast Iso-1-cytochrome c with omega-undecylenyl-beta-D-maltopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.489, 61.438, 74.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.083 - 1.990 |
| R-factor | 0.2073 |
| Rwork | 0.201 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.430 | 37.430 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.259 | 0.054 | 1.819 |
| Rmeas | 0.263 | 0.055 | 1.881 |
| Rpim | 0.044 | 0.011 | 0.473 |
| Total number of observations | 397044 | 7289 | 19036 |
| Number of reflections | 17478 | ||
| <I/σ(I)> | 23.3 | 92.9 | 1.6 |
| Completeness [%] | 99.7 | 98.9 | 98 |
| Redundancy | 22.7 | 30.5 | 15.1 |
| CC(1/2) | 0.998 | 0.999 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Reservior: 90% ammonium sulfate, 0.1 M Tris, pH 7.5 Protein: 8 mg/mL in 75% ammonium sulfate Detergent: 12 mM w-undecylenyl-b-D-maltopyranoside in deionized water Mixed 2:2:1 |
