5KLT

Prototypical P4[M]cNLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.890, 89.550, 99.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.698 - 2.600
R-factor	0.2078
Rwork	0.207
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ul1
RMSD bond length	0.002
RMSD bond angle	0.495
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.7)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.700	26.700	2.720
High resolution limit [Å]	2.600	9.010	2.600
Rmerge	0.300	0.070	1.613
Number of reflections	22383
<I/σ(I)>	9.3
Completeness [%]	99.9	96.4	100
Redundancy	14.1	11.9	14.4
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	296	1M Sodium citrate pH6, 10mM DTT