5KLR
Prototypical P4[R]cNLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-26 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.150, 89.541, 100.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.048 - 2.200 |
| R-factor | 0.1667 |
| Rwork | 0.166 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ul1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.626 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.7) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.050 | 34.050 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.078 | 0.034 | 0.385 |
| Number of reflections | 36898 | ||
| <I/σ(I)> | 25.7 | ||
| Completeness [%] | 100.0 | 98.3 | 100 |
| Redundancy | 14.6 | 11.8 | 14.3 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10mM DTT, 1M sodium citrate pH7.0 |
